Journal: Molecular Cancer Research

Article Title: Mutual Exclusivity Analysis of Genetic and Epigenetic Drivers in Melanoma Identifies a Link Between p14ARF and RARβ Signaling

doi: 10.1158/1541-7786.mcr-13-0006

Figure Lengend Snippet: Figure 3. Cellular responses to ATRA in normal and p14ARF-depleted melanocytes. A, BrdUrd incorporation in primary human melanocytes grown for 48 hours in the presence or absence of ATRA (10 mmol/L) and in the presence or absence of LE135 (1 mmol/L). B, FACS analysis showing representative cell-cycle profiles of normal human melanocytes cultured for 10 days in the presence or absence of ATRA (10 mmol/L). C, ATRA-induced senescence in two cultures of normal human melanocytes (WT-1 and -2) and two independent pools of melanocytes expressing shRNAs against CDKN2A sequences (HEM-1 and -2). Cells were treated with ATRA (10 mmol/L) for 2 weeks, and the number of senescent cells was determined by SA-b-Gal positivity. Columns represent percentage of SA-b-Gal–positive cells. D, the same data as in (C), but with the increase in senescence in normal melanocytes set at 100%. Statistical analysis was conducted using a paired t test. , P < 0.05; ns, nonsignificant.

Article Snippet: LE135 was purchased from Santa Cruz Biotechnology.

Techniques: Cell Culture, Expressing